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For each species at each of the four exposure water changes, we collected a 125 mL sample of each nominal concentration in a precleaned glass amber jar and mixed them together to create 500 mL per exposure level per species.
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Embryos displayed a range of abnormalities in response to MeHg, particularly in brain development, which is influenced by both MeHg concentration and the number of embryos per ml of exposure solution.
We estimated the size of this effect to be a loss of 23 mL in the growth in FEV1 from 5 to 11 years of age per unit increase in PM10 (~ 3.8 mL/year), and 14 mL per unit increase of NO2 exposure (~ 2.3 mL/year).
Duplicate glass dishes containing 10 salmon lice and 200 mL of exposure solution were used per EMB concentration, control (seawater) or solvent control (seawater with 0.01% (v/v) PEG300).
The revealed effective dose per exposure was 0.054 mSv.
S - number of symptomatic per exposure group.
E - number of exposed per exposure group.
Cells were counted using a haemocytometer and seeded at densities of 2 × 105 cells/ml for 3 d of exposure or 5×× 105 cells/ml for 3 h of exposure, respectively in a final volume of 10 ml per 75 cm2 flasks.
Environmental toxicology studies for the most part performed exposure not only in 24-well plates (200 μL to 2 mL per embryo or larva) but also in crystallization dishes, scintillation vials or beakers (450 μL to 5 mL per embryo or larva, 40 to 300 mL per adult), while natural product studies were performed exclusively in multiwell-plate setup (<100 to 250 μL per embryo or larva).
In all, 3 mL Radiesse solution was administered per thigh, 1.5 mL per hemiabdomen, and 1.5 mL per brachial zone.
Suggested volumes are 0.5 1.5 mL per side.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com