Sentence examples for ml per embryo from inspiring English sources

Environmental toxicology studies for the most part performed exposure not only in 24-well plates (200 μL to 2 mL per embryo or larva) but also in crystallization dishes, scintillation vials or beakers (450 μL to 5 mL per embryo or larva, 40 to 300 mL per adult), while natural product studies were performed exclusively in multiwell-plate setup (<100 to 250 μL per embryo or larva).

The minced contents were placed in a 15 ml tube and treated with 0.25% trypsin (0.25% Trypsin/EDTA, Gibco; 1 2 ml per embryo) for 30 min at 37°C, pipetting briefly every 5 min to enhance dissociation.

The embryos were cultured in 1 1 rat serum/GMEM media (1 ml per embryo) supplemented with 200 mM L-glutamine/100 mM sodium pyruvate (Millipore, UK), 25 U/Ml Penicillin/25 µg/Ml Streptomycin, treated or not with 50 μM LY, in rotating wheel incubator at 37°C, 5% CO2.

Decapitated and eviscerated embryos were passed several times through an 18-gauge needle and resuspended on a per embryo basis in 1 ml 10% heat inactivated fetal bovine serum (FBS) in DMEM supplemented with 100 µg/ml DNase and 500 µg/ml collagenase.

Add 1 ml 0.05% trypsin/0.53 mM EDTA per embryo, disaggregate further by pipetting, and incubate 5 min at 37°C.

Plate 1 ml of cells/trypsin into 150 mm tissue culture dishes (one dish per embryo), and try to further disaggregate by pipetting.

Centrosome free extracts were prepared by homogenising the frozen embryos in 2 ml per gram IP buffer (50 mM HEPES pH 7.6, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT, 1× Protease inhibitor cocktail [Roche]) and centrifuging twice at 15,000 RCF.

These publications report a detection limit of 1 or 10 ng/ml of medium, and the quantity of material released was of the order of magnitude from 3 to 80 ng per day and per embryo.

There was no difference between TRT, GRP, or their interaction (P > 0.05) for embryo recovery, embryos recovered, embryo quality, embryo stage, or cells per embryo.

(E) The number of nuclei per embryo was counted under a confocal microscope in embryos stained with DAPI, n = 15 20.

The use of FT semen for insemination did not affect embryo development and embryo quality in terms of total cells number per embryo.