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Particles of carbon nanotubes, at three different levels (0.3, 0.1 and 0.001 %; C1, C2 and C3, respectively) were added to 10 ml of xylene.
In this method, 0.1 g of microcapsules was transferred into dry Gooch crucible with stop cork followed by the addition of 10 mL of xylene with gentle stirring at room temperature.
Briefly, 1 ml of xylene was added to four 20 μm FFPE sections to remove paraffin.
Slices were deparaffinized twice with 1 mL of xylene, vortexed, and centrifuged.
The samples were added with 1 ml of xylene, and then with 1 ml of ethanol.
We mixed 3 mL of the cell suspension with 1 mL of xylene to form a two-phase system.
Similar(38)
Samples were prepared with 5 mL of xylene-based scintillation fluid (Fisher Scientific Co., Fairlawn, NJ).
Heating was continued for a total of 2 h (approximately 3 mL of additional xylene required), then the hot solution was treated with charcoal, filtered through celite and evaporated.
Topical inflammation was instantly induced on the posterior surface of the same ear by application of xylene (0.05 ml).
The paraffin flakes were placed in a 1.5 ml microcentrifuge tube, deparaffinized with 1200 μL of xylene, vortexed, and centrifuged at 16,000 g × 5 min.
Cells were then scraped with a sterile scalpel blade into an Eppendorf tube containing ethanol (1 mL) and centrifuged to remove any trace of xylene.
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