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Approximately 12 ml of whole blood was collected in Tempus™ Blood RNA Tube at enrollment.
At least 10 ml of whole blood was collected by blood draw using EDTA as anticoagulant.
Briefly, 1 2 ml of whole BM marrow or blood was aspirated into a blood gas syringe.
PBMCs were extracted within 2 h of collection from 20 ml of whole blood using Ficoll Plus density gradient centrifugation (GE Healthcare).
Approximately 12.0 mL of whole blood was collected from each patient at the times proposed in the clinical protocol.
Platelet-rich plasma was obtained from 20 mL of whole blood, drawn from the investigator (MG) in lab.
For each patient in the cBMA Group, 30 ml of whole blood was drawn from an antecubital vein.
Aliquots of 11C-LY2428703 (about 150 kBq) were added to 0.5 mL of whole blood or 0.5 mL of plasma.
Now I added half a cup (120 ml) of whole milk and brought it to the simmer.
Next, 1/2 cup (120 ml) of whole milk and a tablespoon of tomato paste out of a tube – it's the milk that makes this such a lush sauce, along with the sausage juices.
3 4 ml of whole blood was collected from each subject.
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