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After hybridization, the microscope slides were washed at 48 °C for 12 min by immersing them into 50 ml of washing solution.
The Ni-resin was washed twice with 10 ml of washing buffer, and His6-PrS2-Bac7 (1–35) witheluted with 20 mM Tris HCl (pH8), 500 mM NaCl and 300 mM imidazole HCl (pH8.0).
Filters were washed twice with 4 ml of washing buffer.
Following two washes with washing buffer the cells were resuspended in 1 mL of washing buffer.
After three washes, the cells were suspended in 1 ml of washing buffer.
After again washing the cells twice, they were suspended in 1 mL of washing buffer for analysis using a FACScan (Becton Dickinson Immunocytometry System, San Jose, CA, USA).
Placental histocultures (18 20 fragments) were exposed to virus stocks containing 15 40×106 genome equivalents in a final volume of 2 ml of washing medium, and in parallel, to washing medium alone (mock infection).
For its corresponding no-AMF control (I-N-AMF), microcosms received 100 ml of washing filtrate from 100 g of AMF inoculum (with no mycorrhizal spores) and equal amounts of inoculum sterilized with γ-radiation to correct for possible differences between the microbial communities in I-AMF and I-N-AMF treatments.
Columns were washed with 10 ml of washing buffer.
The glass milk was washed in 1 ml of washing buffer following manufacturer recommendations.
The IgG beads were washed with 5 ml of washing buffer and centrifuged again.
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