The mixtures were then plated around the rim of wells in a 12-well plate and allowed to solidify in 37 °C incubator for 25 min, followed by addition of 1 ml of warm WIT medium.
The solid was filtered off, and consequently dissolved in 30 ml of warm water (60°C).
On the other hand, different weight of gelatin was dissolved in 25 mL of warm distilled water (40°C).
For gelatin suspension preparation, 1.0 g of gelatin was solubilized into 50 mL of warm distilled water (40°C).
The right lung was lavaged with 6 mL of warm normal saline; then, the recovered BALF were centrifuged at 400 × g for 10 min.
15 g of the crude extract was weighed, dissolved in 100 ml of warm water and poured into inclined separating funnel.
Then the earthworms were removed carefully from the casting materials and transferred to a glass beaker containing 500 ml of warm (40 °C temperature) distilled water and agitated for 5 6 min with a stirrer.
Pulse the machine a couple of times to combine the dry ingredients; then, in a measuring cup, combine 125 ml whole milk with 125 ml of warm water, yielding a mixture that is tepid rather than hot or cold: think of body temperature.
For the inhibitor assays, 1000 cells were resuspended in 2 ml of warm media containing 0.35% agarose, with the appropriate concentration of sulindac, NS398, cyclopamine, tomatidine, or vehicle control.
The initial 25 ml of warm saline injected was discarded.
Trimethoprim sulfadiazine was mixed with approximately 20 mL of warm corn syrup in order facilitate administration.
