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The apparatus requires a 100 ml of test fluid to perform the experiment.
Conclusions: As compared to traditional yogurt, daily consumption of three times 125 ml of test product specifically lowered serum LDL-cholesterol levels in normal healthy male adult subjects with borderline elevated levels of serum total cholesterol within three weeks.
Each group resided in approximately 40 mL of test medium.
5 ml of test solution was added twice daily to the petridish for 6 days.
To 2 mL of test solution 2 mL of freshly prepared DPPH reagent was added.
Each independent newborn toxicity test was carried out in duplicate using seven animals placed in 50 mL of test solution for 96 h [15].
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In the reaction mixture 50 µg/ml of test sample and 1 ml of DPPH (2,2-diphenyl-1-picrylhydrazyl) solution (90 μM) was added and mixture volume was made up to 3 ml.
Various concentrations 5-1000 μg/ml) of test samples were mixed with 2.5 ml phosphate buffer (0.2 M; pH = 6.6) and 2.5 ml potassium ferricyanide (0.03 M).
In this test the inoculum contained each remaining incubation mixture from each well of the micro titer plate where the in vitro test was carried out i.e. 4, 2, 0.4 and 0.1 mg/ml of test substance separately in 0.02 ml of infected blood as described by Yusuf et al.[ 6] and Maikai [ 19].
The various concentrations (1 to 5 mg/ml) of test extract was prepared in 25 ml of H-F salt solution.
The solutions of required concentration (50 μg/mL) of test compounds were prepared by dissolving the compounds in DMSO were filled into the cups with 1 mL of respective solution.
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