Sentence examples for ml of supplemented from inspiring English sources

The overnight culture was diluted 1∶500 in 50 mL of supplemented M9 at 25°C and grown to an OD600 of 0.2, at which time the culture was induced with 15 µM IPTG for 2 hrs.

Beads were washed 3 times in 1 ml of supplemented IP-Buffer before use.

MCF-7 cells were grown to approximately 70% confluence in six-well plates in 2 ml of supplemented EMEM.

Naringenin (200 μM final concentration) was added to 5 × 10 mutant cells in 10 mL of supplemented HL-5.

The PDMS well holding seeded M109 cells was removed and the culture dish was filled with 2 ml of supplemented RPMI medium.

CFU were measured by spreading (in triplicate) 100 µL of different dilutions (10−4, 10−5, 10−6, 10−7, 10−8) of the bacterial cultures in Petri dishes containing 25 mL of supplemented Middlebrook 7H10.

Cells were washed and diluted to OD600 of 0.1 in 50 ml of SD supplemented with 2% galactose and supplemented for auxotrophies.

MGIT tubes were supplemented with 0.8 mL of supplement (BACTEC MGIT Growth Supplement; Becton Dickinson) and were inoculated with 0.1 mL of the drug solution and 0.5 mL of the test strain suspension.

Then, 1 × 10 parental or K-ras transformed E6E7 cells and Capan-1, AsPC-1, Panc-1 cells in 0.5 mL of supplement-free medium with or without 10 μmol/L capsaicin were seeded onto the upper part of each chamber insert, and the 24-well plates were filled with 0.5 mL of their culture medium.

Fermentation was performed in 250 ml shake flasks with 50 ml of medium supplemented with different carbon substrates and grown at 30°C and 180 rpm for 120 h.

A single-colony of each strain was grown in 5 mL of YNB supplemented with d-glucose in a 50 mL conical tube (Sarstedt AG & Co., Nümbrecht, Germany) and incubated at 30 °C and 180 rpm.