Sentence examples for ml of substrate from inspiring English sources

Then, 0.04 ml of substrate (0.075 M acetylthiocholine iodide) was added to the reaction mixture.

Voltammetric responses were recorded in 50 ml of substrate solutions prepared in PBS buffer solution.

The appropriately diluted protein solution was mixed with 0.45 ml of substrate, followed by incubation for 10 min at 50°C.

The assay mixture consisted of 0.4 ml of substrate and 0.1 ml of enzyme solution in 50 mM Tris-HCl buffer pH 8.0.

The lactate dehydrogenase (LDH), as reagent activity of lysate, was measured by addition of 0.3 mL of substrate buffer to the tube.

5 μg of each purified xylanase was incubated with 1 ml of substrate solution, consisting of 1% (w/v) oat spelts xylan in sodium acetate buffer (50 mM, pH 6.0), at each optimal reaction temperature for 10 min, then reaction was terminated by adding 1 ml of 3,5-dinitrosalicylic acid (DNS) and boiled for 10 min.

Finally, the blot was incubated with 5 mL of Chromogenic Substrate solution (BCIP/NBT substrate for alkaline phosphatase) (Invitrogen, USA) until the bands appeared.

At a para-H2 flow rate of approx. 1.5 l/h, roughly 3 4 ml of the substrate evaporated.

4.90 ml of the substrate was taken in flaks marked as test and blank and kept at room temperature for 5 min.

In treatment G CMvw, vermicompost and commercial media mix was supplied with vermiwash and in G CMinor, chemical fertilizer was applied and germination trays were filled with 20 ml of mixed substrate as per treatments in Table 4.

The reaction was initiated by the addition of 0.2 ml crude extract in 4.8 ml of the substrate.