Sentence examples for ml of stop from inspiring English sources

Consecutively, Renilla luciferase activity was measured after adding 100 ml of Stop & Glo Reagent (Promega).

Next, 2.5 ml of Stop Solution was added to stop the reaction.

The resulting suspensions were rapidly filtered through Millipore filter paper (0.65 µm) to retain the vesicles and washed with another 3 mL of stop solution.

The labelling reaction was terminated by transferring the slides to 50 mL of stop buffer (10 mM EDTA, pH 8.0) for 5 minutes.

The substrate was again incubated for 15 min at ambient temperature, and the reaction was stopped by adding 0.1 ml of stop solution (1 M H2SO4).

After 1 h of reincubation, 12.5 mL of stop solution (95% [v/v] ethanol; 5% [v/v] phenol) was added to inhibit cell metabolism [ 121], and the cells were harvested by centrifugation.

The reaction was stopped by the addition of 0.5 ml of stopping buffer (6 M guanidine-HCl, and 1 mM DNTB).

After addition of the enzyme extract, the reaction mixture was incubated at 37°C for 15 min. The reaction was stopped by adding 1 ml of the stop buffer (2.5 g FeCl3 and 6 g trichloroacetic acid in a final volume of 100 ml of 2.5 M HCl).

Cells were then washed with ice-cold phosphate-buffered saline (PBS) containing protease inhibitors, and the fixation reaction was stopped by adding 10 mL of Glycine Stop-Fix Solution (Active Motif).

Cells were stained with the membrane stain carboxyfluorescein diacetate succinimidyl ester (CFSE; 5 μM; ICT, Bloomington, MN, USA) and washed with RPMI supplemented with 10% FBS, 100 U penicillin G per ml, and 100 μg of streptomycin per ml to stop the reaction.

Reactions were carried out at 37°C for 10 min and were stopped by the addition of 3 mL of ice-cold stop solution (0.25 mol/L sucrose, 100 mmol/L NaCl, and 10 mmol/L Tris-HCl, pH 7.4).