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In severe bronchitis the mucous glands lining the bronchi enlarge greatly, and, commonly, 30 to 60 ml of sputum are produced in a 24-hour period, particularly in the first two hours after awakening in the morning.
The deep breathing and coughing procedures were repeated until at least 1 ml of sputum was obtained.
The cornerstone is sputum smear microscopy, a cheap and simple test to detect the presence of acid-fast bacilli in patient's sputum, but which requires a minimum of 10,000 bacteria per ml of sputum, and cannot simply discern drug susceptibilities [1], [5].
An equal amount of bleach was added to 0.5 ml of sputum in a 50 ml Falcon tube, which were vortexed well and incubated at room temperature for 15 minutes, then distilled water was added to a final volume of 50 ml, and inverted 3 4 times.
Patients able to produce at least 1 mL of sputum were enrolled in the colorimetric study and patients with less than 1 mL of sputum or who were unable to produce sputum spontaneously were included in the string test study.
The sample was determined to be adequate if at least 1 ml of sputum was collected into a sterile container.
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The patient was instructed to spit 2 ml of sputum-free saliva into the Oragene DNA Self Collection Kit (DNA Genotek, Ontario, Canada) and stored at room temperature.
A maximum of two attempts were conducted to obtain an adequate sample of at least 3 mls of sputum.
To assess the antimicrobial impact of cysteamine alone and cysteamine in conjunction with antibiotics commonly used to treat infective exacerbations of CF lung disease, 0 · 2 ml of each sputum sample sputum was diluted tenfold in sterile phosphate buffered saline (PBS) and vortexed.
Tunney et al. [ 8] showed that approximately 50% of healthy individuals harbour between 1000 and 10,000 culturable anaerobic bacteria per ml of induced sputum A range of microbial species were also found in induced sputum at low numbers in another study which examined sputum from healthy subjects [ 9].
In another study in adults, 0.72 ml of decontaminated sputum was used for MODS (almost three times higher than our volume) and 0.5 ml was used for MBBacT [7].
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