Swabs were then placed into a 5-ml vial containing 1 ml of specimen transport medium.
HCMV DNA >500 copies per ml of specimen was regarded as positive.
These samples were suspended in 1 ml of specimen transport medium for HPV testing (Digene, Gaithersburg, MD, USA).
For endocervical specimens, 1 ml of specimen lysis buffer was added to endocervical samples, mixed thoroughly by vortexing and were incubated for 10 min at room temperature.
WNV titers were calculated as the tissue culture infectious dose50 (TCID50) per mL of specimen by the method of Reed and Muench (31 ).
In this method, 0.2 mL of specimen was injected into 1 shell vial of R-Mix cells (Diagnostic Hybrids, Athens, OH, USA).
For liquid culture, 1 mL of specimen was decontaminated with N-acetyl l-cysteine/sodium hydroxide (NaOH) 3% and inoculated into MGITs (Becton Dickinson).
A second sample was then obtained with a Qiagen Cervical Sampler (Medscan, Uppsala, Sweden), and suspended in 1 ml of specimen transport medium (STM/Qiagen Inc., Hilden, Germany) for HPV DNA testing.
A second sample was then obtained with a cervical sample device (Digene Inc. Gaithersburg, MD, USA) and suspended in 1 ml of specimen transport medium (STM/ Digene Inc, Gaithersburg, MD, USA) for HPV DNA testing.
