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50 mL of solvent was added to the powder.
The ground leaves were supplemented to a centrifuge tube (eppendrof) containing 10 mL of solvent.
Then 1.5 mL of solvent phase was transferred to the 2 mL auto sampler vial.
The highest percentage oil yield was obtained when 153 ml of solvent was used.
Then 1.25 mL of solvent mixture was poured through filter paper into separating funnel.
The treatment consisted of 20 mg Actilyse in 50 ml of solvent.
5-TP or 6-TH was dissolved in 1 ml of solvent and uploaded in a storage loop (P1).
Briefly, cells were suspended in 5.0 ml of solvent followed by vortexing for 15 s and incubated for 20 min.
Actilyse (20 mg) was dissolved in 100 ml of solvent and administered by a catheter into the pericardium.
The aqueous layer was re-extracted by adding 50 ml of solvent mixture using a similar procedure.
ZnO sol (0.2 M) was obtained by mixing 4.4 g of zinc acetate dihydrate with 100 ml of solvent.
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