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Typical sample concentration is 1-10 mg/mL, and 0.5 mL of solution is required.
After cleavage, the solvent was concentrated under reduced pressure and the remaining ~1 mL of solution was precipitated into an excess of cold diethyl ether.
The target protein was detected in 1 mL of solution at concentrations from 10−10 М to 10−13 М.
At predetermined time intervals, 2 mL of solution was withdrawn, and the amount of 5-FU released from the ZIF-8/5-FU@FA-CHI-5-FAM was examined at 265 nm by recording the UV absorbance of the solution.
The supernatant was discarded and the cell pellet was re-suspended in 2 ml of solution B (400 mM Tris HCl (pH 8.0) 6 0 mM EDTA, 150 mM NaCl and 1% SDS).
1-2 mL of solution is normally sufficient for analysis.
Add 10 mL of solution E to 990 mL of solution F. Agitate to mix thoroughly.
Donor hearts received 1 ml of solution containing 1 x 1010 p.f.u. of adenovirus encoding the beta2-AR or an empty adenovirus as a control.
5 mL of solution has been withdrawn at pre-determined time intervals.
At an interval of 10 min, 3 mL of solution was taken out from the reactor.
Place 1 mL of solution A in about 950 mL distilled water.
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