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Each seedling was inoculated with 1.0 ml of soil solution 2 weeks afterwards.
A volume of 0.1 ml of soil suspension was spread onto nutrient agar plates and incubated at 30 °C for 24 h.
A sample of 5 g soil was suspended in 100 ml water. 1 ml of soil suspension was serially diluted in distilled water.
Fabric soiling was done according to AATCC test method 130. On a bloating paper fabric specimen sized 4″× 4″ was kept and approximately 0.2 ml of soil (mustard oil or hot coffee) was placed on it.
For water extraction of soil phosphate according to Van der Paauw [49] and Murphy and Riley [50], 4.25 ml of soil was suspended in demineralised water for approximately 22 h.
We collected ≈200 mL of soil from this location at depths of up to ≈15 cm from the surface.
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Data of the probes indicated that field capacity conditions corresponded to a soil water content around 50 ml water/100 ml of freshweight soil, while the water stress treatment reduced soil water content until ~13 ml water/100 ml soil (Table 1).
After centrifugation, two aliquots of 1 ml of the soil pore water were each mixed with 4 ml Ultima Gold XR and the 14C-radioactivity was measured in a liquid scintillation counter (Tricarb 2800TR, PerkinElmer, Waltham, USA).
A total of 0.1 mL of the soil suspensions without formaldehyde solution was inoculated in triplicate on both liquid and solid (1.5% agar) mediums of BG11 (Stanier et al., 1971) and NC (Kuhl, 1962), respectively.
Ants were placed, in pairs, in plastic coffee cups (diameter 45 mm) with Fluon-coated sides containing approximately 10 ml of nest soil and a ball of moist cotton.
Eight days after transplanting, 4 ml of field soil suspension was added to each individual plant.
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