Sentence examples for ml of soft from inspiring English sources

It was placed in a sterile Petri dish, overlaid with 10 ml of soft agar (0.8%) containing test strain S. aureus (about 106 cells/ml).

The bottom layer consisted of 10 ml of 1.5% LB broth agar (Amresco, USA) on which the inhibitor-producing test strain grew, and the top layer consisted of 6 ml of soft 0.7% agar (Helicon, Russia) containing 1% tryptone, 0.5% yeast extract (Oxoid Ltd., UK) and 0.1% NaCl, to support the growth of the indicator strain (M. catarrhalis).

Briefly, MRS agar plates containing 15 ml of MRS agar were overlaid with 8 ml of soft agar (0.8%) containing 50 μl of freshly grown culture, and the plates were incubated at 4°C for the soft agar media to set.

Prepoured MRS agar plates were overlaid with 10 ml of soft MRS agar inoculated with 0.3 ml of an overnight culture of the indicator organism grown in M17 broth at 37°C.

Cells were counted and 5×103 SAOS-2 and CD133 and SAOS-2 cells were plated in 6-well culture dishes in 3 mL of soft agar growth medium; 500 µL of medium per well were added every 2 days.

Trypticase soy agar plates were overlaid with 5 mL of soft agar (0.75%) containing 50  μL of freshly grown culture.

Briefly, a top agar was prepared containing 1 ml of phage solution and 1 ml of a bacterial overnight culture in 30 ml of soft-agar.

Briefly, 25 ml of soft-agar (heated at 45°C) was inoculated with 0.1%% of an overnight culture of the indicator strain L. ivanovii HBP28, poured into a Petri dish and allowed to set for 30 min at room temperature.

Afterwards, a suspension with a viable count of 1 × 10 CFU/mL was prepared; 200  μL of this suspension was added to a tempered tube containing 7 mL of soft-agar Mueller Hinton (Lab. Britania; Argentina) and distributed evenly in Petri dishes with 13 mL of Mueller Hinton agar (Lab. Britania; Argentina, 15.5 g/L).

Cells were harvested by the 10 µl microbiological loop and suspended in 300 µl of Brain Heart Infusion broth (BD, USA) to 1 MF, and then 100 µl of the indicator strain culture was added to the 6 ml of the soft agar and gently stirred.

Bacteria were grown as described above, suspended in LB (2×105 CFU/ml), mixed with 3 ml of 0.7% soft agar (Sigma) and poured on Petri dishes containing 10 ml LB agar.