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After purging nitrogen at 100 °C for 30 min, the reaction mixture was cooled to 65 °C, and 0.1 mL of sodium methoxide (0.5 mmol, 27 mg in 30wt% methanol) and 3 mL of anhydrous tetrahydrofuran (THF) were added.
Subsequently, 6 mL of sodium citrate aqueous solution was injected again.
To this solution, 100 ml of sodium selenosulfate solution was added drop by drop.
Afterwards, 44.5 mL of sodium hydroxide solution (10 M) is added to the solution.
Then 0.75 mL of sodium bicarbonate (60 mg/mL) solution was added and mixed thoroughly.
A procoagulant effect was reported previously after injection of 0.50 to 0.75 ml of sodium hyaluronate (Healon) into the vitreous cavity at the end of phakic diabetic vitrectomies.
Compound 2 was obtained by adding 100 mL of sodium hydroxide solution to 300 mL of castor oil.
Crude tannase (2000 Units/ml) was slowly mixed with 10 ml of sodium alginate (5 %) by avoiding air bubbles.
The mixture was heated to boiling, and then 6 mL of sodium citrate aqueous solution (1 wt%) was injected quickly.
To these solutions, 250 μL of Folin-Ciocalteu reagent (1 N) and 1.25 mL of sodium carbonate (20%) were added.
Then 1.4 ml of sodium silicate was added to the suspension to precipitate the silica coating on pigment.
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