Total DNA was extracted from 1 ml of root, shoot or seed extract using DNeasy Plant Mini Kits (Qiagen, USA), and eluted in water.
The hypocotyl segments were placed upright, with the basal end embedded in 100 × 25 mm Petri dishes containing approx. 40 ml of any of three different shoot induction media (IM1, IM2, and IM3).
CM-BD1 (1 mL L 1) also resulted in higher values, although without statistical difference, of shoot and root dry weight (SDW, RDW), N content and total N in shoots (NC, TNS).
The extraction of lipid peroxides was carried out using 500 mg of fresh shoot tissue, 0.3 mL of 10% trichloroacetic acid, and 1 mL of 0.5% TBA.
At each harvest, root exudates were collected (see next section), shoot dry weight was recorded, and the shoot P concentration was determined after digestion of 0.3 g of finely ground shoot material in 4 mL of HNO3 and 2 mL of H2O2 under pressure at 175°C for 1 h in a microwave oven.
Lipase was exacted from 4 g frozen tissue powder from 15 shoots with 20 ml of 0.2 M phosphate buffer (pH 7.8) containing 0.05 M mercaptoethanol for 1 min at 4°C [28].
100 g of sliced bamboo shoot was boiled in 300 mL of double distilled water for 2 h at 100°C.
For analysis of LOX activity, 4 g of frozen tissue powder from 15 shoots was homogenized in 20 ml of phosphate buffer (pH 7.0).
