Sentence examples for ml of serum from inspiring English sources

Untransfected cells and cells transfected with HIF-1α/shRNA1470, HIF-1α/shRNA2192 or HIF-1α/shRNASCR (a scrambled negative control) were covered with 1 ml of serum free DMEM media and placed in hypoxic conditions (0.5% O2) for 8 h.

Each column was washed with 5 ml of serum, and the residual radioactivity of the washed adsorbents was evaluated.

Consequently, 100 μL of MTT dye (5 mg in 10 ml of serum free medium) added in each well.

A serum sample of a healthy volunteer was stored frozen until assay. 2 mL of methanol was added to 1.5 mL of serum sample.

1.0 mL of serum was spiked with a NLX standard drug solution to contain 1.0×10−1 mol L−1 and deprotinated by adding 1.0 mL acetonitrile.

A final volume of 1 ml of serum was used.

Untreated and treated PC12-ND6 cells were incubated in 1 ml of serum-free medium containing 3 µl of the fluorescent DNA dye PicoGreen® (Invitrogen) for 1 h at 37 °C.

Two hundred viable cells of C. albicans were cultured in 24-well flat tissue culture plates containing 1 ml of serum-free DMEM in a 37 °C/5% CO2 incubator.

As soon as cells started dislocating from the surface, the flask was rinsed with 5 mL of serum-containing medium to arrest the trypsinization.

Cells were cultured at 37°C in 5% CO2, rinsed twice, and pre-incubated for 1 h with 2 ml of serum-free medium at 37°C.

The cells were incubated for 48 hours in 0.4 ml of serum-free medium.