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The optimum values for the factors were found to be 0.06 mL of seed, 7.2 × 10−3 M of ascorbic acid and 0.05 M of the CTAB in the final solution.
At this point, 0.8 mL of seed solution were added to the growth solution and the resulting mixture was stirred.
A 250-ml shaker flask containing 30 ml of seed medium was inoculated with 3 ml of mycelial solution.
The inoculum was incubated in a 250-mL Erlenmeyer flask containing 50 mL of seed medium at 30 °C and was shaken at 180 rpm for 25 h.
Firstly, 10 mL of "seed" solution was prepared by mixing CTAB (1 mmol) and HAuCl4∙3H2O (2.5 × 10−3 mmol) at room temperature.
Large-scale culture was performed by transferring a-10 mL of seed culture into an 1-L Erlenmeyer flask containing 250 mL of PDB and incubated at 130 rpm and 28 °C for 10 days.
Similar(31)
After the seed culture, 2 ml of the seed culture broth were inoculated into 25 ml fermentation medium in 250 ml Erlenmeyer flasks, which were incubated at 30 °C with 180 rpm for 6 days in the batch culture.
5 mL of the seed inoculum was added into the 50 mL fermentation broth.
About 6 mL of each seed extract was dissolved in 6 mL of methanolic solution of DPPH (100 µM).
The final step was the addition of 0.02 mL of the seed solution to the growth solution.
For monacolin K production and gene expression testing, 5 ml of the seed medium was inoculated into two types of fermentation medium (50 ml).
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