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For analysis, 0.1 mL of samples was diluted to 1 mL using nanopure water.
During the reduction, 0.1 ml of samples was taken and diluted several times with millipore water.
To 0.5 mL of samples of each concentration it was added 0.5 mL of 0.2 M phosphate buffer pH 6.6.
At specific time intervals, 5 mL of samples was withdrawn and immediately replaced with the same amount of fresh media.
Briefly, 50 mL of samples was pipetted into milliliter beaker, and mixed with 1 mL of the salicylate solution.
At predetermined time intervals, 2 ml of samples were withdrawn from the dissolution medium and immediately replaced by the same volume of fresh medium.
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Filter approximately 1 mL of sample into a HPLC Vial.
5 ml of sample was collected and analyzed.
In this method, 2.0 mL of sample is placed in contact with 4.0 mL of acetonitrile.
Under optimum conditions, a preconcentration factor of 200 was obtained with 10 ml of sample.
The proposed system reached an enrichment factor of 50, with 5 mL of sample.
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