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In this method, 2.0 mL of sample is placed in contact with 4.0 mL of acetonitrile.
Under optimum conditions, a preconcentration factor of 200 was obtained with 10 ml of sample.
The proposed system reached an enrichment factor of 50, with 5 mL of sample.
Starting from 1 mL of sample, the nucleic acids were eluted into a final volume of 50 μL and stored at −80 °C.
Disposable plastic cuvettes were used with 1.0 mL of sample solution, which was filtered through a 200 nm filter immediately before measurement.
The virus pellet was collected by centrifugation (Sorvall SLA 1500 rotor, 29,753 × g, 40 min) and suspended in about 10 ml of sample buffer containing 20 mM Tris acidic buffer (pH 6) and 3% (wt/vol) NaCl.
Filter approximately 1 mL of sample into a HPLC Vial.
5 ml of sample was collected and analyzed.
Initially, 0.6 ml of sample (2000 ppm) was mixed with 2.34 mL of DPPH solution.
(B) Sample application: Add a maximum of 1.0 mL of sample to the column.
0.1% (Sharma et al. 2012) C. Take 1 mL of sample milk in a test tube.
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