Treatment consisted of 1,000 IU hCG or 1 mL of saline (control) administered i.m.
PET image acquisition started immediately with intravenous administration of FDG through the T connector cap (flushed with 0.5 mL of saline).
Bioluminescence was measured before inoculation (baseline), after inoculation and after irrigation with 6 ml of saline using luminometer.
A 10-ml aliquot of defibrinated blood and 10 ml of saline were mixed and centrifuged.
Afterwards, the fungal colonies were transferred to tubes containing 5 mL of saline solution (9 % NaCl).
The BTX-A was dissolved in 2 mL of saline to 50 U/mL before use.
One stone cube can take up approximately 50 ml of saline solution.
- Aspirate 20-25 ml of saline from the IV bag into the syringe.
For protocols B, C, and D, 50 ml of saline was injected at 2.5 ml/s, 5 ml/s, and 10 ml/s, respectively.
Then, 1 ml of stock was diluted with 1 ml of saline to 62.5 U/μl urokinase solution, and 1 ml of 62.5 U/μl urokinase solution was diluted with 1 ml of saline to 31.25 U/μl urokinase solution.
A needle of adequate length is used and botulinum toxin is diluted with at least 2 ml of saline solution.
