Alkali copper reagent 50 ml of reagent a + 2 ml of reagent b).
Four milliliters of sample solution was added to 1 ml reagent A. To this mixture 1 ml of reagent B was added.
A 0.3 mL extract was combined with 3 mL of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate).
(b) CuSO4 solution (0.5 mg CuSO4, 5H20 + 100 ml of 1 % sodium potassium tartrate solution) (c) Alkali copper reagent 50 ml of reagent a + 2 ml of reagent b) (d) Folin reagent solution (folin reagent:distilled water = 1 1 (e) 1 (N) NaOH solution (f) Standard BSA solution of concentration 200 mg/l.
(b) CuSO4 solution (0.5 mg CuSO4, 5H20 + 100 ml of 1 % sodium potassium tartrate solution) (c) Alkali copper reagent 50 ml of reagent a + 2 ml of reagent b) (d) Folin reagent solution (folin reagent:distilled water = 1 1 (e) 1 (N) NaOH solution (f) Standard BSA solution of concentration 200 mg/l. . 5%Na2CO3solutionion.
An aliquot of 100 µl of sample solution was combined in an Eppendorf tube with 1 ml of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate).
The sample and solution were taken in a ratio of 1 20 in 125 mL of reagent bottle and kept at 30 ± 2 rpm for 18 h at room temperature.
A 5 mL solution containing 0.5 to 2.5 mg dextrose units was pipetted into test tubes and 5 mL of reagent was added; then, the solution with the added reagent was mixed well by stirring.
An aliquot of 100 µl of each sample was mixed with 1 ml of reagent (0.6 M H2SO4, 0.028 M sodium phosphate, and 0.004 M ammonium molybdate) and incubated for 90 min at 95 °C in a water bath.
A typical blank solution contained 1 ml of reagent solution and the appropriate volume of the solvent used for the sample, and it was incubated under the same conditions as the rest of the samples.
An aliquot of 0.1 ml of each fraction (dissolved in respective solvent) was combined in a vial with 1 ml of reagent solution (0.6 M sulphuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate).
