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The cassette was washed with 100 mL of purified water.
A 250 mg of azithromycin reference standard and nanosized azithromycin powder were, respectively, added into different vessels containing 900 ml of purified water.
The pelleted cells were resuspended (at a concentration of approximately 3 × 109 cells/ml) in 1 ml of purified X. oryzae pv.
The reaction mixture contained 0.1 mL of purified recombinant bromelain and 1.1 mL of 1% casein in 0.1 M Tris-HCl buffer (pH 8.0) with 20 mM cysteine (final concentration).
A 1-carboxymethy-3-methylimidazolium chloride ([CMMIM]·Cl, Figure1A) stock solution (1.0 mmol·L−1) was prepared by dissolving 0.0875 g of [CMMIM]·Cl (Shanghai Shyfhx Reagent, Shanghai, China) in 500 mL of purified water.
A 1-aminoethyl-3-methylimidazolium bromide ([AEMIM]·Br, Figure1B) stock solution (2.0 mmol·L−1) was prepared by dissolving 0.01 g of [AEMIM]·Br (Shanghai Shyfhx Reagent, Shanghai, China) in 25 mL of purified water.
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8 mL (1.91 U/mL) of purified lipase from Bacillus aerius was then incubated with the matrix for 1 h under shaking condition.
A 6-well plate was coated with 1 ml formaldehyde fixed S. aureus (1×109 cell/ml) or 50 µg/ml of purified protein A. The plate was incubated at 37°C with 5% CO2 for 2hrs.
A dose of 1 mg/ml of purified bioflocculant was optimal for the clarification of Kaolin suspension (100 ml) following Jar test.
100 μM of each EDC was incubated for 1 h at 25°C in a reaction mixture containing 1.5 U/mL of purified laccase in 50 mM sodium citrate buffer, pH 5.0; total reaction volume was set to 4 mL.
This cell-free protein synthesis procedure produced 412 μg/mL of purified GLuc, relative to 5 μg/mL isolated for intracellular Escherichia coli expression.
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