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Then, 1 ml of hydrochloric acid was added, after which 25 ml of potassium sulphate solution was also added.
Animals were euthanized with a bolus infusion of 10 ml of potassium chloride 19.1% at the end of the experiment.
Then, 0.5 mL of supernatant was mixed with 0.5 mL of potassium phosphate buffer (10 mM, pH 7.0).
A volume of 2 mL sample solution was added into 2.5 mL phosphate buffer (0.2 M, pH 6.6) and 2.5 mL of potassium ferricyanide (1%, w/v).
The reaction mixture contained 1.6 ml of supernatant, 0.4 ml of 50% TCA, 0.4 ml of ferrous ammonium sulphate and 0.2 ml of potassium thiocynate.
When the reaction pH was not 7, the pH was corrected to 7 with 0.1 mL of potassium phosphate buffer 1 M (pH 7) before an absorbance measurement.
For the anthocyanin and flavonoid content assays, the sample extracts in 2 mL of potassium phosphate buffer (100 mM, pH 7.8) were ground into powder with liquid nitrogen.
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The cells were filtered and washed with K10 buffer before being suspended in 5 mL of potassium-free K0 buffer.
Mix the content thoroughly and add 0.5 ml of 10% potassium chromate solution.
The animal was then euthanized with a high dose of pentobarbital followed by 100 ml of 2M potassium chloride solution.
Subsequently, we added 2 mL of methanolic potassium hydroxide, stirred for 1 min and heated to 56 °C for 20 min.
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