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Two 10 ml syringes were used one containing 9 ml of physiologic saline (0.9% NaCl) solution and the other containing 1 ml of air.
To avoid dehydration, mice scored with 6 were subcutaneously given 0.5 ml of physiologic saline solution.
In the control group, the solution was 25 ml of physiologic saline.
Control mice were i.p. injected with 0.2 ml of physiologic saline.
After washing the cartridge, the desired radiotracer was eluted with 10 mL of physiologic saline solution.
The solution was allowed to have an effect for 15 s, before 5 mL of physiologic saline solution was used to flush the area.
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The lungs were excised, bronchial lavage samples were collected for analysis of cellular content via a tracheal instillation and withdrawal (repeated 3 times) of 7 mls of physiologic saline, and type II cells isolated using an elastase digestion technique [24].
Hitherto, the specimens were rinsed with physiologic saline from outside, gently dried and immediately put into a new centrifuge tube (tube 1) with 20 ml of fresh physiologic saline, and the time was measured beginning with this starting point (time = 0 min in Fig. 3).
Before starting the procedure, 20 ml of sterile physiologic saline was instilled into the joint cavity.
About 100 mL of sterile physiologic saline warmed to the body temperature was instilled in 20 mL aliquots.
Four soil samples of ≈5 mL each were suspended in a total volume of 20 mL of sterile physiologic saline (0.85% NaCl).
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