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Gemcitabine (900 mg m−2) was dissolved in 20 ml of physical saline and then diluted further with physical saline or 5% glucose to a volume of 250 ml.
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Very thick ices were obtained in this way (60 500 ML) and a range of physical conditions was determined where observations were best reproduced: the so-called favorable zone.
DNA was extracted from 1 mL of culture broth using a physical disruption method (MORA-EXTRACT, AMR, Gifu, Japan) according to the manufacturer's instructions, with a final DNA elution volume of 200 400 μL.
Cells were re-suspended in 1 mL of sterile PBS and lysed by physical disruption using the MiniBeadbeater-8™ (BioSpec Products , Inc.
In lieu of physical education.
Partly because of physical proximity.
–Possibility of physical transition.
A lot of physical biochemistry.
Optimization of physical parameters and medium components showed an overall 3.2-fold increase in activity compared to unoptimized condition (179.06 U/ml).
Lack of physical verification.
Adiponectin showed no difference with levels of physical activity (low 9.2 ± 4.3 μg/ml and high 10.0 ± 5.0 μg/ml; P = 0.29) or with differences in diet habits.
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CEO of Professional Science Editing for Scientists @ prosciediting.com