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To 25 g of each sample, 225 ml of peptone water was added and homogenized.
The effect of nitrogen source on both mycelial growth and melanin production was observed with the highest mycelial biomass achieved in the medium containing 1.0 g/100 mL of peptone for A. fumigatus AFGRD105.
A loopful of isolated colonies was inoculated into 4 ml of peptone water, incubated at 37°C for 4 h.
The large intestines were aseptically removed after 5 days of re-nutrition, weighed and placed into sterile tubes containing 5 ml of peptone water (0.1%).
Ammonia production was assayed by inoculation of bacterial strains in 10 mL of peptone water and using Nessler's reagent (0.5 mL).
Three to five morphologically similar colonies of each organism (aged 18 24 h) were touched with a sterile loop and inoculated in 4 ml of peptone water (BDH Chemicals Ltd) prepared according to manufacturer's recommendations.
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Ten grams of soil from each composite sample was added to 90 mL of sterile peptone water (bacto peptone, 1 g/L) and homogenised by stirring at 130 rpm for 10 min (dilutions of 10−1 to 10−8).
The 24 h inoculums were prepared in 250 mL wide-necked Erlenmeyer flasks containing 100 mL of yeast peptone dextrose medium (YPD) composed of 20 g/L glucose, 5 g/L peptone, and 5 g/L yeast extract.
Salad in each sterile bag was then mixed thoroughly with 225 ml of buffered peptone water.
Standard plate counts were performed after decimal dilution of the samples in 9 ml of 0.1% peptone water.
The samples were put in a sterile universal bottle filled with 225 ml of buffered peptone water.
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