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The amount of bleeding was 1200 mL and total intraoperative transfusion comprised 2240 mL of packed red blood cells, 1920 mL of FFP, and 200 mL of packed platelets.
Another 30 ml/kg of lactated Ringer's solution and 560 ml of packed red blood cells (four units) were given.
Because she was hemodynamically unstable, we added another 840 ml of packed red blood cells (six units).
Besides crystalloid solution, 1200 ml of fresh frozen plasma (FFP), 1400 ml of packed red blood cells, and 350 ml of albumin were administered.
A total of 2500 ml of crystalloid and 800 ml of colloid were administered, and 840 ml of packed red blood cells, 1680 ml of fresh frozen plasma, and 400 ml of platelets were transfused.
The overview of resuscitation is shown in the Fig. 2. The patient received 2520 ml of packed red blood cells, 1200 ml of fresh frozen plasma, 200 ml of platelet and 2600 ml of intraoperative blood salvage, in addition to 5350 ml of fluid administration.
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Once the loading solution cooled to ~0°C, 5 mls of packed RBCs were added in a single injection.
Briefly, a 10 mL volume of packed erythrocytes was washed with RPMI (Invitrogen) twice; then a 200 μL volume of the packed erythrocytes was thoroughly mixed with 800 μL of ice cold 1% Triton X-100 (Sigma-Aldrich Co. LLC) in TBS buffer solution (Wako) and a proteinase inhibitor (Complete H; Roche Diagnostics, Mannheim, Germany) was included in each tube (total 1 mL).
TPMT activity was normalized per milliliter of packed red blood cells (Units/mL).
After packed erythrocytes were washed three times in 10 volumes of phosphate-buffered saline, 0.5 ml of the packed cells were diluted to 5 ml with phosphate-buffered saline containing l mmol/l ferricyanide and incubated under magnetic stirring in a water bath at 37°C.
Mice were housed individually and fed diets as described above in a paste (225 g of powdered chow as described above with 45 ml of water) packed into 50-ml plastic tubes to facilitate weighing of food intake.
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