Different dosage ratio of 1 25, 1 20, 1 15, 1 10, and 1 5 which were 60, 75, 100, 150, and 300 mL of anaerobic organisms were filled in five containers, respectively, and kept under ambient air for 24 days.
Then, anaerobic organisms were added to leachate at ratio of 1 10 (150 mL of anaerobic organism) using measuring cylinder.
In another work, using anaerobic organisms in a series of anaerobic tanks filled with leachate, 100 mL of anaerobic organism and 14 days of microbial inhibitors, 65.5, 60.2 and 46.3 % of COD, NH3 N and color were removed, respectively (Kamaruddin et al. 2013).
Therefore, based on the observation from the figure, the highest removal of COD concentration achieved when 100 mL of anaerobic organism was introduced in the leachate sample.
The experimental results also showed that the the optimum variables condition was established at pH 7 of leachate sample, dosage of 100 mL of anaerobic organism and required 14 days to reduce the COD, NH3 N and colour pollutants to 65.5, 60.2 and 46.3 %, respectively.
Diluted inoculums (0.1 mL) of test organism (10 cfu/mL) were spread on Muller-Hinton agar plates.
Briefly, the second stage suspension cultures (25 mL) of all experimental organisms were added with racemic Carvedilol solution in methanol (0.01 mg/mL culture).
When none of the equids became ill or seroconverted by 30 days post exposure, they were challenged intravenously with 5 ml of heavy suspension of organisms.
One ml of each indicator organism (5 × 105 cfu ml-1) was inoculated into 15 ml of semisolid BHI or MRS agar (BHI or MRS broth plus 0.7% bacteriological agar) maintained at 50°C and then poured into a petri dish.
One ml of each test organism liquid culture was individually suspended in 3 ml of a 0.9% NaCl solution.
A 1 10 dilution of each hand rub was produced by adding 1 ml of the challenge organism suspension to 9 g of the hand rub.
