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0.3 g of dried rabbit brain powder,4.9 ml of normal saline, and 0.1 ml of 0.1 mol/L sodium oxalate were vibrated in 45°C water bath.
After 6 h of incubation, 1 mL of normal growth medium containing twice the normal serum concentration was added without removing the transfection mixture.
Another one group of animals received only 2 ml of normal saline per testis as a control.
All drugs were injected as an intravenous bolus followed by 20 ml of normal saline.
We used 6,000 ml of normal saline with a fluid deficit of 500 ml approximately.
18F-FLT (2.96 MBq/kg) in 2−5 mL of normal saline was injected intravenously.
Then, 0.1 mL of normal saline suspension of revived bacteria was added to each test tube.
We performed FC with 1,000 ml of normal saline solution infused in 30 minutes.
Cefazolin 1 g with 100 ml of normal saline and about 300 ml of normal saline were given via peripheral venous catheter during operation, and intravenous fluid via peripheral venous catheter was stopped after induction of cardiopulmonary bypass.
The right middle lobe was lavaged with 60 mL of normal saline to collect bronchoalveolar lavage fluid (BALF).
The cystogram was performed using 250 ml of iodinated contrast diluted with 100 ml of normal saline.
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