Well in short, lots—I learned that I'm terrible under pressure, I learned that a constant inhaling of 0.7 mls of nicotine in the space of three hours will give me the shakes, and I learned that saying 'no' to teenagers is enough of a reason for something to become the height of cool.
Nicotine was administered in a gum (4 mg of nicotine).
Standard textbooks, databases, and safety sheets consistently state that the lethal dose for adults is 60 mg or less (30 60 mg), leading to safety warnings that ingestion of five cigarettes or 10 ml of a dilute nicotine-containing solution could kill an adult.
There was a further threat under discussion: the treatment of e-cigarettes that deliver more than 20mg of nicotine per ml as medical rather than consumer products.
Second hand smoke was estimated by sampling of vapor-phase nicotine using an air pump (model HB3344-02 Tuff Personal Air Sampling, produced by Casella Cell, Badford, UK) coupled with an impinger (Pyrex glass Midget Impinger, from SKC Ltd ,Dorset, UK) filled with 15 mL solvent (usually acetonitrile) for extraction/desolvation of nicotine from pumped air.
We used a high speed of injection (200 msec), previously shown to facilitate nicotine self-administration and the volume of each injection was 0.2 ml for the 10 µg/kg/injection dose of nicotine.
Nicotine nasal spray is an aqueous solution of nicotine in a 10 mL spray bottle [ 23].
Exposure to high concentrations of nicotine, such as that found in a 30 mL bottle of 36 mg/mL "cotton candy" flavored nicotine liquid used to refill an e-cigarette, can be toxic.
