Sentence examples for ml of minimal from inspiring English sources

Batch tests were carried out in 1-L glass bottles with 200 mL of minimal medium.

Therefore, 30 50 mg of cells harvested in the late exponential growth phase were resuspended in 2 mL of minimal media.

Seed cultures of yeast cells were grown during 20 24 hours in shake flasks containing 25 ml of minimal medium at 30°C and constant shaking (120 rpm).

For submerged fermentation, 100 ml of minimal media was placed in a 500-ml Erlenmeyer flask supplemented with 1%% (w/v) rice straw.

Yeast cell seed cultures were grown in 25 ml of minimal medium supplemented with 20 g/l of glucose, and incubated at 30°C for 22 24 hours (130 rpm).

Approximately 5 g of soil collected from both mangroves were inoculated in 200 mL of minimal medium MM (peptone 0.3%, K2HPO4 0.05%, MgSO4 0.05%) containing sugar cane bagasse 3% as the carbon source.

The yeast strain was inoculated in to 50 ml (lab scale), 150 ml (PDU) of minimal medium contained in a 150 ml (lab scale) and 300 ml (PDU) shake flasks, respectively; incubated at 30°C on an orbital shaker at 180 rpm for 24 h.

For the isolation of G418-resistant transformants, cells were suspended in 1 ml of MA5 minimal medium, spread onto MA5 minimal agar plates containing 100 μg ml−1 G418 and 1 mM uracil, and incubated in a CO2 incubator at 5%% (v/ v) CO2 and 25 °C under fluorescence tubes at 50 μmol photon m−2 s−1.

The cellulolytic ability of each isolate was tested by inoculation into 3 mL tubes of minimal medium with CMC plus sugarcane bagasse, pinus (Pinus elliottii) powder, or medium-density fiberboard (MDF) powder at 1%, as the main carbon source.

For cultures in PHB production medium, cells from rich media precultures were washed once with a sterile 0.85% NaCl solution and used to inoculate a 200 mL culture of minimal medium enriched with 2% fructose and 0.05% NH4Cl to an initial OD600 of 0.05 in a 1 L culture flask.

For the growth assay in liquid culture, each overnight culture cultivated in a test tube containing 3 mL of M9 minimal medium was diluted 1 100 with fresh M9 minimal medium to obtain a total volume of 3 mL, before growing overnight in test tubes with agitation.