Exact(6)
The cells were washed with ice-cold PBS and incubated on ice for 6 min with 1.0 ml of mild acid (0.2 M acetic acid – 0.5 M NaCl, pH 2.5).
(9.8 ml) of mild dishwashing liquid.
Add 2 tablespoons (30 ml) of mild detergent to one of the buckets.
Add roughly 1 Tbsp (15 ml) of mild dishwashing detergent to the water.
Mix 1/8 cup (25 ml) of mild detergent in 1 gallon (3.78 liters) of water, or follow the directions on the slate cleaner container.
Combine 1 to 2 Tbsp (15 to 30 mL) of mild dish detergent with 1 gallon (4 L) warm water in a large bucket.
Similar(54)
Briefly, 100 mg of PCL/Pluronic F68 compound and 17.65 mg of docetaxel were dissolved in 100 mL of acetone by mild heating and sonication.
In a typical procedure, 1.5 g of NaTA was added to 150 mL of H2O under mild stirring with the assistance of sonication, and the pH of the solution was adjusted to 1.6 with hydrochloric acid.
After local District Governmental Animal Investigation Committee approval, blood volume was altered in both directions randomly in steps of 150 ml (mild) to 450 ml (moderate) either by haemorrhage, retransfusion of blood, or infusion of colloids in six Foxhound dogs.
The duration of surgery was 4 h and 57 min, the duration of anesthesia was 6 h and 29 min, the volume of blood loss was negligible, the in-out balance was + 3210 mL (crystalloid fluid 4175 mL, urine volume 965 mL), and mild swelling of the face and both upper limbs was noted.
Activity on Avicel (PH 101, Fluka, Buchs, Switzerland) at 3.5 g/L was performed similarly (final volume of 4 mL) under mild shaking (70 rpm) at 37°C at a final enzyme or complex concentration of 0.1 µM except that 800-µL aliquots were pipetted at specific intervals and centrifuged for 10 min (10,000 g) at 4°C.
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