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Lithium acetate dihydrate (1.02 g, 10 mmol) in 10 mL of MeOH was added drop-wise to a solution of purpurin (2.56 g, 10 mmol) dissolved in 100 mL of methanol.
Beyond 2 mL of MeOH, the dispersion phenomenon leads to a decrease in yields.
After 2 h the absorbance was measured at 760 nm (blank prepared in the same way, 0.5 ml of MeOH instead of standard solution).
A solution of compound 4 (220 mg, 0.354 μmol) in 3 mL of MeOH was stirred with 10 mg of Pd/C under H2 atmosphere for 12 h.
After loading, cartridges were rinsed with 5 mL of H2O to wash out any salts and vacuum-dried at 0.68 bar for 5 min. Cartridges were eluted with 2 × 1.25 mL of MeOH and 2 × 1.25 mL of MeCN.
Prior to use, individual stock solutions containing 100 mg mL-1 of (C) and (G) were prepared by extracting 10 g of each reference materials using 100 mL of MeOH for 10 min in a pear-shaped separating flask.
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A volume of 1.5 ml of MeOH-Water (1 1) was added to the callus samples.
A volume of 1.5 ml of MeOH-Water (1 1) was added to the samples.
One hundred milligrams of seeds were homogenized using a mortar and pestle with 5 mL of MeOH/CHCl3 (2 : 1, v/v) and 1 mg PDA (pentadecanoic acid in MeOH) as an internal standard.
The solution is evaporated in a drying oven at 110 °C, the residue is dissolved in 0.5 ml of MeOH/THF (1 1), and then filtered.
Washed radiolabeled cells were suspended in 4 ml of MeOH/100 mM Tris HCl (pH 7.4) containing 4 mM MgCl2, 2∶1.
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