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Wash with 10 ml of medium.
After 60 minutes, the inoculum was replaced by 50 ml of medium without FBS.
Cells were plated in quadruplicate wells containing 100 ml of medium and GdCl3.
Then, 1 ml of medium was added to each chamber with the cells facing up.
Placental barrier integrity was measured with an EVOM-2 voltohmeter and a STX3 Ag/AgCl electrode (World Precision Instruments Ltd) containing 3 mL of medium on the baso-lateral and 0.5 ml of medium on the apical transwell side.
Hepatocyte monocultures were seeded at a density of 0.6 × 106 viable cells in 1.6 mL of medium per well.
Meanwhile, 3.5 mL of medium with high glucose but without A23187 was added into the wells (n = 6 in each group).
BM content was flushed directly into 5 mL of medium (Dulbecco's modified Eagle's medium GlutaMAX™, 10 mM HEPES, 10% foetal bovine serum) using a syringe.
Dry weight was recorded as mg per ml of medium (mg/ml).
Glass bottle (650 ml capacity), each having 100 ml of medium was used as culture vessel.
The reactions were performed in a 25-ml jacketed reactor with 10 ml of medium system.
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CEO of Professional Science Editing for Scientists @ prosciediting.com