Your English writing platform
Discover LudwigExact(60)
1.5 (high tolerance) glass-bottomed dishes (MatTek Corporation, USA) in 2 ml of media.
After this layer had solidified, 2 ml of media (media, 10% FBS) was added.
1.5 glass-bottomed dishes were seeded with 1 × 105 CHO cells expressing wtEGFR in 2 ml of media with 250 ng/ml of doxycycline (~4 × 105 receptors/cell).
injections of 1 × 108 cells in 1 mL of media.
All cultures were carried out in 250 mL conical flasks, in 50 mL of media.
Aerobic growth cultures were performed in 50 mL conical tubes containing 5 mL of media.
250 µl of the siRNA TransIT-siQUEST mix wasiRNA TransIT-siQUESTcontaining 1.25 mixof media washout addediotocs.
Cells were grown on 75-cm2 cell-culture flasks with 10 mL of media that was changed every second day.
25 ml of media was added to each petri dish and allowed to solidify for 20 min in laminar air flow.
injections in the flank with 2 × 106 cells in 0.2 mL of media containing 20% Matrigel ™(Becton Dickinson, Bedford, MA, USA) or intraperitoneal (i.p).
To count, cells were washed with Hanks, trypsinized, centrifuged, and resuspended in 5 ml of media and then counted manually using Haemocytometer every day for 7 days.
More suggestions(1)
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com