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Mycelia were grown in 100 mL of malt extract broth (20 g/L Difco malt extract) on a rotary shaker at 120 rpm for 6 8 weeks.
The culture was carried out in Erlenmeyer flasks with 50 mL of malt extract liquid media, 200 ppm of dye and an inoculum of four cylinders from the fungus.
The fungus BCC 22389 was fermented in a 1000 mL Erlenmeyer flask containing 250 mL of malt extract broth (MEB; malt extract 6.0 g/L, yeast extract 1.2 g/L, maltose 1.8 g/L, dextrose 6.0 g/L) at 25 °C for 38 days under static conditions.
For all experimental crosses, the parental male and female were placed in a plastic vial (8 mL of malt medium) when mature (age 13 26 days from eclosion).
The populations were established and maintained at the same density of five pairs per bottle (containing 50 mL of malt medium), with constant population size and nonoverlapping generations.
Each generation the parental pairs were allowed to mate and lay eggs for 10 days in plastic vials containing 8 mL of malt medium.
Similar(51)
Standing liquid culture of A. mellea mycelium was performed at 25 26 °C for 3 weeks in polypropylene bottles (1.2 l) for mushroom cultivation filled with 800 ml of saccharified malt medium (11 Brix°).
To prepare uniform inocula, fungi were grown in 100 ml of yeast malt (YM) broth as described [ 10, 26].
The spore suspensions were added to the 250-mL Erlenmeyer flasks containing 50 ml of nutrient media (Malt Extract Broth).
All genotypes were collected in Italy and grown for ten days in multi-well culture plates filled with 2 ml of 2 % Malt Extract Agar (MEA) before being harvested.
(10 ml) of milk.
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