Sentence examples for ml of ice from inspiring English sources

3 4 hours following biotinylated protein injection, mice were perfused transcardially with 20 ml of ice cold 1X DPBS.

The cross-linked cells were washed in 1 ml of ice cold HBSS containing a protease inhibitor cocktail (PIC, 200x; final concentration 1x; Sigma-Aldrich, P8340).

The cell pellet was resuspended in 500 μl of PBS and fixed by adding 4.5 ml of ice cold 70% ethanol.

Ten mL of ice cold sodium hydroxide (0.5 M) were added to stop the reaction after 30 min incubation.

Briefly, mice were euthanized with isoflurane and perfused transcardially with 25 ml of ice cold PBS.

At selected time points animals were sacrificed and their peritoneal cavities were lavaged with 1 ml of ice- cold PBS.

Pellet was washed twice with 0.5 ml of ice-cold acetone.

5 minutes following tomato lectin injection, mice were perfused transcardially with 20 ml of ice-cold 1X DPBS followed by 20 ml of ice-cold 4% PFA in 1X DPBS.

Four intermittent consecutive boli consisting of 10 mL of ice-cold saline were randomly injected over the ventilatory cycle.

The cells were then centrifuged at 1,500 g for 5 min at 4 °C and resuspended with 40 ml of ice-cold sterile water, centrifuged at 1,500 g for 5 min at 4 °C and resuspended with 20 ml of ice-cold sterile water, centrifuged at 1,500 g for 5 min at 4 °C and resuspended in 20 ml of ice-cold 1 M sorbitol, and centrifuged at 1,500 g for 5 min at 4 °C and resuspended in 0.5 ml of ice-cold 1 M sorbitol.

Filters were washed three times with 3 ml of ice-cold KRH buffer containing pyrocatechol using a Brandel cell harvester (model M-48; Brandel Inc., Gaithersburg, MD).