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Fluid absorption of over 1000 ml of hypotonic solution can cause clinical hyponatraemia.
[D] Fluid absorption of over 1000 ml of hypotonic solution can cause clinical hyponatraemia.
A small volume (generally 10 15 mL) of hypotonic fluid such as sterile water or 1.5%% glycine was infused through the device for acoustic coupling.
The Guideline Development Group's consensus view is that once a fluid deficit of 1000 ml of hypotonic solution or 2500 ml with an isotonic solution is reached immediate suspension of the procedure is imperative.
The experiment sample comprised of stock of mice erythrocyte (RBC) suspension (0.50 mL) with 5 mL of hypotonic solution (50 mM NaCl) in 10 mM sodium phosphate-buffered saline (pH 7.4) containing either the different methanolic extracts (2.0 mg mL−1) or acetyl salicylic acid (0.10 mg mL− 1).
The final pellet was resuspended in 1 ml of hypotonic buffer.
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[D] Mild symptoms can develop even with absorption of 500 1000 ml of a hypotonic solution.
[D] Mild symptoms can develop even with absorption of over 500 ml of a hypotonic solution.
The cell pellets were resuspended in 25 ml of a hypotonic buffer (20 mM HEPES pH 7.8, 1 mM MgCl2, 1 mM PMSF and 10 µM leupeptin) and allowed to swell on ice for 10 minutes.
The cell pellets were lysed with 0.5 mL of a hypotonic solution containing 10 mM Tris HCl, pH 7.4, 1 mM EDTA, and 0.2% Triton X-100.
Aliquots of 0.5 ml of the hypotonic cell suspensions were centrifuged (Cytospin 2, Shandom) onto clean glasses at 800 rpm for four minutes, and fixed in -20°C methanol for 30 minutes.
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