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1.0 ml of human plasma was added to each tube.
were extracted from 0.5 ml of human plasma by acetonitrile protein precipitation.
Extraction involved ion-paired liquid liquid extraction of the compounds from 1.0 ml of human plasma adjusted to pH 7.0.
Best thawing conditions were found to be: 4 °C, with 0.25 mL of human plasma and ultrasound (US) thawing.
The spiked human urine was prepared by adding a 30 mL of 10 ppm of GA solution to a 30 mL of human urine.
5.0 mL of human blood sample was taken by using heperanized tube after an average of 6 hrs following the last oral administration and centrifuged at 3000 rpm for 30 minute.
Similar(32)
To obtain dendritic cells (DCs), monocytes were treated with 1,000 U/ml of human recombinant granulocyte macrophage-colony stimulating factor (Peprotech Inc).
Fig. 1 Cell survival of TNF sensitive L929s cells following exposure to 0 300 U/mL of human TNF solutions and preincubated with 250, 50 and 10 ng/ml 99mTc-S-HYNIC CZP.
Cells were cultured in 2 ml per well of CM (containing 6000 IU/ml of human recombinant IL-2) in a 37°C, 5% CO2, humidified incubator.
Culture dishes were coated overnight at 4°C with 40 µg/ml of human fibronectin in a final volume of 10 ml in PBS and subsequently blocked with 1% fatty acid-free BSA in PBS, 1 h at room temperature.
During the quantitation experiment, we added a fixed concentration of 10 ng/ml of human chorionic gonadotropin (hCG) protein to act as an interferer.
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