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For these experiments, the quartz dewar used to collect the polarized frozen beads was substituted by a glass vial filled with 3 ml of hot D2O, preheated to 100 °C and placed at the centre of a 100 mT permanent magnet.
This was dissolved in 5 mL of hot methanol.
Again, crystals were dissolved in 5 ml of hot acetone to crystallize at room temperature.
The crucibles were then emptied into 400 ml beakers containing 100 ml of hot water.
After 4 h of vigorous stirring, the suspension was filtered and washed with 250 ml of hot water.
About (0.03079 g) of (AMPY) was dissolved in 10 mL of hot water and added to the reaction mixture.
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The residue was washed with two 5 mL portions of hot 2% HCl.
After 30 min, 200 ml of a hot aqueous solution that contained 0.0626 g of barium carbonate was added to the hot solution that contained magnesium and vanadium.
After 72 h the DES was dissolved while shaking by adding 1 ml of 70 °C hot ddH2O until full dissolution of the DES was reached.
For the synthesis of the barium doped catalyst (BaVMgO), 700 ml of a hot aqueous solution that contained 1.030 g of ammonium metavanadate was added to 4.6427 g of MgO.
For determination of endogenous NA and DMA concentrations in the T1 plant body, approximately 5 g of frozen shoots and roots from each transgenic and NT line was ground and homogenized with a mortar and pestle and then suspended in 100 ml of deionized hot water (80 °C) for 20 min.
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