Sentence examples for ml of homogenization from inspiring English sources

Briefly, brains were washed in PBS containing 0.25 M sucrose and homogenized using a Dounce Homogenizer in 1 mL of homogenization buffer (containing 300 mM sucrose, 5 mM EDTA, 5 mM HEPES, 1 µM phenylmethanesulfonylfluoride (PMSF) and protease inhibitors, pH 7.4).

The sections were minced and homogenized with 2 ml of homogenization buffer per gram of tissue, using a Teflon-glass homogenizer.

To extract high-molecular weight genomic DNA, each aliquot of embryos was homogenized in a Dounce 15-ml homogenizer in 10 ml of homogenization buffer.

Each ear was homogenized in 1 ml of homogenization buffer (10 mM KCl, 1.5 mM MgCl2 and 10 mM Tris-HCl [pH 7.4]) on ice as described [78].

Tissues were collected and frozen on dry ice and stored at −80°C. 100 mg of liver or muscle was homogenized in 1 mL of homogenization buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 25 mM β-glycerophosphate, 20 mM sodium fluoride, 1 mM sodium orthovanadate, 2 mM sodium pyrophosphate, 2 mM EDTA, and Roche Complete protease inhibitor cocktail) using a TissueLyser II (Qiagen, Valencia, CA).

Brain samples (0.5 g) from patients with AD, PSP and CBD were each homogenized in 10 ml of homogenization buffer (HB: 10 mM Tris HCl, pH 7.5 containing 0.8 M NaCl, 1 mM EGTA, 1 mM dithiothreitol).

Immediately before homogenization, one tablet of [Protease Inhibitor Cocktail (PIC), Roche] was dissolved in 50 mL of the homogenization buffer.

Briefly, the HeLa cells (2×107 cells) were harvested, rinsed twice with ice-cold PBS, and resuspended in 1 ml of a homogenization buffer (20 mM HEPES (pH 7.5), 0.5 mM EDTA, 0.5 mM EGTA, 2 mM MgCl2, 25 mM KCl, 1 mM AEBSF, 1 mM Na3VO4, 5 mM NaF, 5 µg/ml aprotinin, and 5 µg/ml leupeptin) containing 0.25 M sucrose.

MEFs were homogenized in 1.2 ml of ice-cold homogenization buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% Triton X-100, 1 mM PMSF, complete protease inhibitor cocktail (Roche) and phosphatase inhibitors).

The method of 2-△△Ct was used to analyze the real-time PCR results and gene mRNA levels were expressed as the fold change relative to the mean value of control group.> 100 mg frozen colonic mucosa tissue was minced and homogenized in 1 mL of ice-cold homogenization buffer RIPA containing the protease inhibitor cocktail Complete EDTA-free (Roche, Penz-berg, Germany).

For homogenization, the cell pellet was resuspended in 1.5 ml of 1X isosmotic homogenization buffer supplemented with protease inhibitor cocktail and transferred into a glass tubes for the homogenizer (Braun, Melsungen, Germany).