Sentence examples for ml of growing from inspiring English sources

Bacteria from 3 ml of growing cultures (1 5 × 10 bacteria ml−1) of HB19 or B31 A3 strains and derivatives were subjected to centrifugation for 30 min at 1500 × g at ambient temperature (room temperature, RT).

Approximately 20 seeds were evenly sown per 9 cm Petri plate (Bibby Sterilin Ltd) containing 20 mL of growing medium, and plates sealed with Parafilm® laboratory film (Pechiney Plastic Packaging, Menasha, USA).

The mini-type bioreactor containing 0.2 L of the P2 medium was inoculated with 20 mL of growing cells (~16 h) and then maintained at 37°C and pH 5.0, by the addition of 2 N NH4OH, and agitated at 150 rpm for 24 h until butanol titer in the fermentation broth was over 5.0 g/L.

For photomicrography, we placed 1 ml of growing cells into a 35-mm petri dish with a poly- d-lysine-coated glass bottom (MatTek Corp).; after the cells had settled onto the surface, they were visualized using an Applied Precision PersonalDV live cell imaging system.

Vegetative colonies and asexual reproduction were observed by examining a small aliquot of cells grown continuously by inoculating ~0.5 1.0 mL of actively growing cells into fresh medium every 3 10 days.

For directed evolution of M. anisopliae, the tubing was filled with Sabouraud dextrose (SAB) media and autoclaved prior to use. 2 mL of a growing culture of M. anisopliae 2575 grown in SAB was injected into the first section of the growth chamber and dilution cycles were initiated as described.

Aliquots (10 mL) of exponentially growing cells were transferred to pre-heated conical flasks of replicates of each strain.

Lysates deriving from 50 ml of exponentially growing cells were enriched for Yox1p-HA as indicated above.

The viruses were amplified by adding the bacteriophage to 500 mL of exponentially growing Synechococcus at a virus to host ratio of 1 [14].

In summary, 45 ml of exponentially growing cells were treated with formaldehyde (37% v/v) for 30 min, to 1% final concentration for DNA-protein crosslinking.

The eluted phage was subsequently used to infect 10 ml of exponentially growing E.coli TG1 at OD600 = 0.5 for 35 min at 37°C.