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Human skin fibroblasts (100,000/ dish) were added on top of the gel in 3 ml of full EGM-2 media.
Tissue was collected by centrifugation and resuspended in 10 ml antitrypsin (Ovalbumin 0.7 mg/ml, Sigma), centrifuged and resuspended in 2 ml of full NSC media.
In a separate experiment, 3 × 10 DU145 cells were resuspended in 1 ml of full media per sterile universal container.
In a separate experiment, 5000 DU145 cells were resuspended in 1 ml of full medium in sterile universal containers.
Amounts of 5 ml of full medium was then added to make up a final volume of 6 ml.
In total, 5000 DU145 cells were seeded out in T25 flasks in 6 ml of full media.
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Rice plants were transferred into appropriate 120 × 14 × 10 mm plastic vessels, containing 12 mL of full-strength Kimura B nutrient solution.
T. roseum cultures were grown in 120-ml crimp-capped serum bottles with butyl rubber stoppers containing 20 ml of full-strength T. roseum medium.
Essentially as described earlier [30], [31] bacteria were grown to mid-logarithmic phase in 10 ml of full-strength (3% w/v) trypticase soy broth (TSB) (Becton-Dickinson, Cockeysville, USA).
As previously described [58], [59], bacteria were grown to mid-logarithmic phase in 10 ml of full-strength (3% w/v) trypticase soy broth (TSB) (Becton-Dickinson, Cockeysville, USA).
Essentially as described earlier [28], bacteria were grown overnight in 10 ml of full-strength (3% w/v) trypticase soy broth (TSB) (Becton-Dickinson, Cockeysville, MD), whereas fungi were grown in YPD medium and washed twice with 10 mM Tris, pH 7.4.
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