Sentence examples for ml of freezing from inspiring English sources

Resuspend the pellet from each flask in 3 mL of freezing solution.

The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS).

The lymphocytes were then isolated by the Ficoll gradient technique and suspended in 2 mL of freezing media containing 10% dimethyl sulfoxide, 40% RPMI 1640 medium, 50% fetal bovine serum, and 1% antibiotic/antimycotic.

The part of ascending colon tissue used for viable counts was placed in 3 mL of freezing media (0.85 g/L di-potassium hydrogen phosphate, 0.2 g/L potassium dihydrogen phosphate, 0.6 g/L tri-sodium citrate dihydrate, 0.25 g/L magnesium sulfate heptahydrate, 121 mL 99.5% glycerol, 879 mL distilled water).

The lower limit for linear quantitation for this assay was 100 copies/ml, which corresponds to 100 copies/swab (swabs were placed in 1 ml of freezing media).

For freezing and long-term storage the proliferating cells were harvested from the tissue culture flask and resuspended in 1 mL of freezing medium (DMEM, 10% Dimethyl sulfoxide (DMSO), 20% Fetal Calf Serum) at 4°C.

Rose Bengal Plate Test (Central Veterinary Laboratory, UK), was used for screening sera from lactating cattle and goats in which milk was sampled for Brucella DNA detection as described previously, [ 57]; and the results were confirmed by c-ELISA as per the protocol described by AHVLA U.K. Volumes of 15 ML of frozen milk sample was thawed and centrifuged at 14,000 G for 10mn.

As cells approached confluency, the growth medium was aspirated from the cells and 2-4 mls of freezing solution were applied.

A total of 720 ml of freshly frozen plasma (six units) and 200 ml of platelets (ten units) was given during TAE.

The resulting solution was then dried on a rotary evaporator, mixed with 5 mL of H2O, frozen at −78 °C and subsequently lyophilized overnight.

Cells were then scraped into 300  μl of lysis buffer (10 m M Tris pH 7.5, 150 m M NaCl, 0.25% NP40, with one protease inhibitor tablet (Roche, Welwyn Garden City, UK) per 10.0 ml of buffer), frozen, thawed, and lysed for 30 min at 4 °C.