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Cells with phagocytised beads were mixed in 200 μL of a solution including 50 mL of flow cytometric solution, Hematall (Fisher, Montreal, Canada) mixed with 100 mg of sodium azide and 0.250 mL of formaldehyde.
After this, 2.5 mL 5x fixation reagent (46 mL of 0.5 M potassium phosphate and 54 mL of formaldehyde) was added to 10 mL cultures of cells.
A 1% denaturing agarose gel with 18% (v/v) formaldehyde in 1xMOPS (0.2 M MOPS (pH 7.0), 80 mM NaOAc, 10 mM EDTA) was prepared by melting 80 mg agarose in 57.7 ml of water prior to the addition of 8 ml of 10xMOPS and 14.3 ml of formaldehyde and cast.
Rats were fixed in Bouin solution (750 mL of saturated picric acid, 250 mL of formaldehyde 37%, and 50 mL of glacial acetic acid) or 4% paraformaldehyde (PFA) using vascular perfusion, and the samples were dissected and post-fixed by immersion (12 h).
A culture of 500 ml of fission yeast cells was grown to OD 0.5 at 32°C and crosslinked with 7 ml of formaldehyde 37% for 20 min at 25°C, 60 rpm.
Embryos were fixed in Bodian's fluid [90 ml of ethanol (80%), 5 ml of acetic acid (99%) and 5 ml of formaldehyde (37%)] and prepared for scanning electron microscopy.
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After vigorously stirring for 20 min, 400 mg of resorcinol and 0.56 mL of formaldehyde-water solution (37 wt.%) were added into the very diluted mixture and stirred overnight, to coat a resorcinol-formaldehyde (RF) resin layer on the surface of nano-Si/SiOx sample.
A volume of 3 ml of 37% formaldehyde was added to achieve a final formaldehyde concentration of 12.3%.
In a 100 mL beaker, 10 mL of 37% formaldehyde (123 mmol) was added slowly to a mixture of 18.6 g of aniline (200 mmol) and 6 mL of concentrated HCl and kept in the water bath at 80 °C for 2 h with intermittent stirring.
Cells were pelleted, washed briefly in 200 µl of 0.1% SDS and incubated in 1 ml of 5% formaldehyde at room temperature for 30 minutes.
Bacteria (1 × 10 CFU/ml) were fixed in 1 ml of 3% formaldehyde for 2 hr then washed with PBS.
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