Your English writing platform
Discover LudwigSuggestions(5)
Exact(40)
After centrifugation and a washing step with deionized water, the pellet was resuspended with 20 mL of fermentation medium, and immediately used for inoculation of the fermenter.
Aliquots (2 mL) of fermentation broth were collected from the bottles.
Fermentations were conducted at 30°C in 250-mL Erlenmeyer flasks with 100 mL of fermentation medium.
The effect of oxygen was investigated using 25 ml bottles containing 10 ml of fermentation broth, which were incubated at 120 rpm and 37°C.
For CDW determination, 100 ml of fermentation medium was centrifuged (7745×g, 10 min), and the pellet was washed with 0.7%% (w/v) NaCl.
Shake-flask fermentation was performed by inoculating 7-mL seed culture in a 250-mL flask containing 50 mL of fermentation medium.
Similar(20)
A 5.0 ml sample of fermentation broth (including biomass) was diluted with 5.0 ml isopropanol (LC-MS grade, Sigma-Aldrich), placed in an ultrasonication bath for 20 min, and centrifuged at 4000 × g for 5 min. A 1ml subsample was transferred to a 2 ml HPLC vial.
For the analysis of substrate consumption and product formation, 1 ml of the fermentation broth was collected throughout the fermentation and centrifuged for 5 minutes at 9000 × g.
Overnight culture of R. toruloides grown in YPD fermentation media (2% w/w glucose) was used to inoculate 50 ml of the fermentation media with an optical density (OD600) of 0.2.
For enzyme production, the submerged fermentation (SmF) was carried out in 250-mL Erlenmeyer flasks with 70 mL of the fermentation medium.
Samples comprising 100 mL of the fermentation broth were taken periodically for further examination.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com