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We mixed 1 mL of extract solution with 1 mL of 2% methanolic AlCl3*6H2O.
The reaction mixture contained 2 ml of Bradford reagent, 16 ml of 0.15 M NaCl and 40 ml of extract.
From the supernatant, 1 mL of extract was pipetted and 4 mL of anthrone reagent was added to each tube.
Plant extract samples were prepared under the same conditions by using 2 ml of extract instead of rutin.
The reaction mixture containing 1 ml of riboflavin, 1 ml of methionine, 1 ml NBT, and 50 ml of extract.
One ml of extract was taken in a tube and 3 ml of BSA solution was added and kept for 15 min at room temperature.
Similar(18)
Results were expressed in μM Fe (II)/mL of extract and compared with that of BHT.
Total RNA was isolated using 1.5 mL of Extract-all Reagent® (Eurobio AbCys, Courtaboeuf, France) per 50 mg of gonad powder, according to the manufacturer's instructions.
A solution of DPPH (0.3 mM) in ethanol was prepared and 1 ml added to different concentrations 5-1000 μg/ml) of extract samples.
The free radical scavenging activity (antioxidant activity) of the extract was evaluated using DPPH radical and it was found that the IC50 occurred at 96.21 ± 9.03 μg/ml of extract.
Hwang et al. (2010) reported that, freeze dried hot water extract from Sargassum hemiphyllum contained 0.240 mg phenolic compound/ml of extract.
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